1. Quartic Polynomial
2. Rational functions 
3. Enzyme Kinetic and Lineweaver-Burk plot
4. Square Root functions

Example 1: Let us solve the following equation:

Solution: We factor this equation into the following:

Thus, the solution is given by x = -2, -1, 1, and 2. 

Example 2: Let us solve the following equation:

Solution: Provided x is not 6, we can multiply through by x - 6 giving:

Thus, x = 4 or 5.

Example 3: Consider the function

Find the domain of the function and the x and y-intercepts. Determine any vertical or horizontal asymptotes. Sketch the graph of this function.

Solution:

• The denominator is zero when x = 3, so the domain is all real numbers, such that x ¹ 3
• The y-intercept satisfies x = 0, so y = 2/(-3) = -2/3
• The x-intercept is found by solving (x+2)/(x-3) = 0 or x + 2 = 0, so x = -2
• Since the degree of the numerator is equal to the degree of the denominator, there is a horizontal asymptote
• For large x, we can neglect the constants in the numerator and the denominator, so

• Thus we have a horizontal asymptote at y = 1
• The denominator is zero at x = 3, so there is a vertical asymptote at x = 3

Example 4: Consider the function

Find the domain of the function. Determine any vertical or horizontal asymptotes. Sketch the graph of this function.

Solution:

• The domain of the function is found from the denominator equaling zero

• The domain is all real numbers such that x ¹ ±2
• The values excluded from the domain represent vertical asymptotes, giving two vertical asymptotes at x = -2 and x = 2
• Since the degree of the numerator (2) is equal to that of the denominator, we have a horizontal asymptote
• Letting x approach positive or negative infinity, we can ignore the 4 in the denominator to obtain:

Example (Lineweaver-Burk Plot): The Michaelis-Menten rate function traces out a hyperbola with the velocity function as

The inverse of this expression is

Let y = 1/V and x = 1/[S], then

The slope of this line is K_m/V_max, the y-intercept is 1/V_max, and the x-intercept is -1/K_m. Below is a graph of this straight line, the Lineweaver-Burk plot.

Thus, by graphing 1/V versus1/[S], with data on an enzyme (forming a line), the Lineweaver-Burk allows easy identification of the Michaelis-Menten constant K_m and the maximum velocity V_max.

Example 5: Suppose an enzyme satisfies the equation

a. Sketch a graph of this function for 0 < [S] < 100. Find any horizontal asymptotes.

b. Determine the linear equation for x = 1/[S] and y = 1/V, then create the Lineweaver-Burk plot. Find the intercepts on the x and y axes. What is the slope for this line?

Solution:

a.

• Since the degree of the numerator (1) is equal to that of the denominator, we have a horizontal asymptote
• As S approaches positive infinity, we can ignore the 10 in the denominator, and we find a horizontal asymptote at y = 20

b. For x = 1/[S] and y = 1/V, we have [S] = 1/x and V = 1/y. Substituting these into the given equation, and solving for y, we obtain

or

 

The slope of this line is 1/2, and the y-intercept is 1/20. The x-intercept is found by setting y = 0, and solving for x. Therefore, the x-intercept is equal to -1/10.

Example 6: Consider the following functions with square roots. Find the domains and ranges of these functions, then sketch their graphs.

Solution:

a. The quantity under the square root sign must be positive, so to find the domain we must first solve

Thus, the domain is x < 4. The range can be seen from the graph below and satisfies y > 0.

b. The quantity under the square root sign must be positive.

• The domain satisfies

8 - 2x - x² > 0

• First solve

8 - 2x - x² = (4 + x)(2 - x) = 0

or

x = -4 and 2

for the boundary of the domain

• The domain is given by -4 < x < 2. (This quadratic function is positive in this interval and negative outside this interval.)
• The largest value under the radical occurs when x = -1 with a value of 9
• Thus, the range of the function is given by 0 < y < 3.
• This graph is a semi-circle